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Research |
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Sliding movements of myosin filaments on actin filaments come to muscle contractions and cell movements. These movements are coupled with free energy emitting process in ATP hydrolysis at the motor domain on myosin head. We investigate the molecular mechanisms and regulatory processes of these events by techniques of protein chemistry, molecular biology and cell biology. We are also interested in the significance of intracellular Ca2+ and the relationship between its concentration and protein phosphorylation. |
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Myosin Phosphorylation and Regulatory Mechanisms of Smooth Muscle Contraction |
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We presented the functional role of the essential light chains in intramolecular interaction between myosin two heads for phosphorylation-dependent regulation. |
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Physiological Roles of Non-muscle Myosin Isoforms |
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1. Non-muscle cell movements involve dynamic transition processes where myosin II assembles into filaments and disassembles into monomers. We identified the essential regions for filament formation and proposed molecular models.
2. We began a research to controle fiblofrast proliferation and shape alteration by using silicon substrate with micro fabrication on its surface. We found some clues to elucidate the molecular mechanisms of the interaction between microfilaments including myosin and actin and adhesion plaques. |
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Regulatory Mechanisms of Calcium / Calmodlin |
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1. We determined Ca2+-free structure of yeast calmodulin by NMR, and proposed the molecular model that 3 mol of Ca2+ cations are bound cooperatively.
2. We identified genomic structure of the sponge, Halichondria okadai, Ca2+-binding protein, calcyphosine gene.
3. We discovered a novel protein CaNBP75 which specifically interacts with Ca2+-dependent protein phosphatase, calcineurin. |
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Articles |
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| ‚‹´³sA’†‘ò—²ŽjA²“¡HA•½“c®–çAŽO‹´–œ—œŽqA–îàV“¹¶
(2006) |
| ”ñ‹Ø×–Eƒ~ƒIƒVƒ“IIƒAƒCƒ\ƒtƒH[ƒ€‚Ì‹@”\
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| ¶•¨•¨— 46(1), 15-19
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| Hagiwara, S. Takahashi, M. Shen, Y. Kaihou, S. Tomiyama, T. Yazawa, M.
Shin, Y. Tamai, Y. Kazusaka, A. and Terazawa, M. (2005) |
| A Phytochemical in an Edible Tamogi-take Mushroom (Pleurotus
cornucopiae), D-mannitol, inhibits ACE Activity and Lowers the Blood
pressure of Spontaneously Hypertensive Rats. |
| Biosci. Biotech. Biochem. 69(8), 1603-1605
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| (PMID:16116292) |
| Nakasawa T., Takahashi M., Matsuzawa F., Aikawa S., Togashi Y., Saitoh T., Yamagishi A., and Yazawa M., (2005) |
| Critical Region for Assembly of Vertebrate Nonmuscle Myosin II. |
| Biochemistry 44, 174-183 |
| (PMID:15628858) |
| Moriya M., Ochiai M., Yuasa HJ., Suzuki N., and Yazawa M., (2004) |
| Identification of Ca2+-Dependent Calmodulin-Binding Protein in Rat Spermatogenic Cells as Complexes of the Heat-Shock Proteins. |
| Mol. Reprod. Dev. 69, 316-324 |
| (PMID:15349844) |
| Nakatomi A. and Yazawa M., (2003) |
| Identification and Characterization of a Novel Calcineurin-Binding Protein in Scallop Testis. |
| J. Biochem. (Tokyo) 133(2), 159-164 |
| (PMID:12761177) |
| Yokouchi, T., Nogami, H., Izumi, Y., Yoshino, H., Nakashima, K., and Yazawa, M., (2003) |
| Solution X-ray scattering data show structural differences among chimeras of yeast and chicken calmodulin: implications for structure and function. |
| Biochemistry 42(7), 2195-2201 |
| (PMID:12590609) |
| Ishida H, Nakashima K, Kumaki Y, Nakata M, Hikichi K, and Yazawa M. (2002) |
| The solution structure of apocalmodulin from Saccharomyces cerevisiae implies a mechanism for its unique Ca2+ binding property. |
| Biochemistry 41(52), 15536-15542 |
| (PMID:12501182) |
| Yuasa HJ, Nakatomi A, Suzuki T, and Yazawa M. (2002) |
| Genomic structure of the sponge, Halichondria okadai calcyphosine gene. |
| Gene 298(1), 21-7 |
| (PMID:12406572) |
| Ishida H, Nakashima K, Kumaki Y, Nakata M, Hikichi K, and Yazawa M. (2002) |
| 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state. |
| J. Biomol. NMR 23(4), 323-324 |
| (PMID:12398353) |
| Katoh T, Konishi K, Yazawa M. (2002) |
| Essential light chain modulates phosphorylation-dependent regulation of smooth muscle myosin. |
| J. Biochem. (Tokyo) 131(5), 641-645 |
| (PMID:11983069) |
| Yazawa M. (2002) |
| Quantitative analysis of Ca(2+)-binding by flow dialysis. |
| Methods Mol. Biol. 173, 3-14 |
| (PMID:11859770) |
| Saitoh, T., Takemura, S., Ueda, K., Hosoya, H., Nagayama, M., Haga, H., Kawabata, K., Yamagishi, A., and Takahashi, M., (2001) |
| Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts. |
| FEBS Lett. 509, 365-369 |
| (PMID:11749957) |
| Yuasa HJ, Suzuki T, Yazawa M. (2001) |
| Structural organization of lower marine nonvertebrate calmodulin genes. |
| Gene 279(2), 205-212 |
| (PMID:11733145) |
| Hayashi Y, Senba S, Yazawa M, Brautigan DL, Eto M. (2001) |
| Defining the structural determinants and a potential mechanism for inhibition of myosin phosphatase by the protein kinase C-potentiated inhibitor protein of 17 kDa. |
| J. Biol. Chem. 276(43), 39858-39863 |
| (PMID:11517233) |
| Numata T., Katoh T. and Yazawa M. (2001) |
| Functional role of the c-terminal domain of smooth muscle myosin light chain kinase on the phosphorylation of smooth muscle myosin |
| J. Biochem. 129, 437-444 |
| (PMID:11226884) |
| Konishi K, Kojima Si, Katoh T, Yazawa M, Kato K, Fujiwara K, and Onishi H. (2001) |
| Two New Modes of Smooth Muscle Myosin Regulation by the Interaction between the Two Regulatory Light Chains, and by the S2 Domain |
| J. Biochem. 129, 365-372 |
| (PMID:11226875) |
| Hagiwara, S., Takahashi, M., and Yamagishi, A. (2001) |
| Species differences in the distribution of the nonmuscle myosin heavy chain IIB inserted isoform in the brain. |
| Zool. Sci. 18, 43-49 |
| (PMID:) |
| Takahashi M, Takahashi K, Hiratsuka Y, Uchida K, Yamagishi A, Uyeda TQ, Yazawa M. (2001) |
| Functional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II |
| J. Biol. Chem. 276, 1034-1040 |
| (PMID:11042201) |
| Ishida H, Takahashi K, Nakashima K, Kumaki Y, Nakata M, Hikichi K, and Yazawa M. (2000) |
| Solution structures of the N-terminal domain of yeast calmodulin: Ca2+-dependent conformational change and its functional implication |
| Biochemistry 39, 13660-13668 |
| (PMID:11076504) |
| Miyazaki, T. Watanabe, M. Yamagishi, A and Takahashi, M. (2000) |
| B2 exon splicing of nonmuscle myosin heavy chain IIB is differently regulated in developing and adult rat brain. |
| Neurosci. Res. 37, 299-306 |
| (PMID:10958978) |
| Uryu M, Nakatomi A, Watanabe M, Hatsuse R, Yazawa M. (2000) |
| Molecular cloning of cDNA encoding two subunits of calcineurin from scallop testis: demonstration of stage-specific expression during maturation of the testis |
| J. Biochem. 127, 739-746 |
| (PMID:10788781) |
| Takahashi, M. Hirano, T. Uchida, K and Yamagishi, A. (1999) |
| Developmentally regulated expression of a nonmuscle myosin heavy chain IIB inserted ieoform in rat brain. |
| Biochem. Biophys. Res. Commun. 259, 29-33 |
| (PMID:10334910) |
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