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第25回 生命分子化学セミナー |
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| 『Distinct p53 Genomic Binding Patterns in Normal and Cancer-derived Human Cells』 |
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| 講演者 |
Dr. Carl W. Anderson
Brookhaven National Laboratory, USA |
| 日時 |
平成２３年１０月２５日（火） 15:00 - |
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| 場所 |
北海道大学理学部 2号館402室 |
| 要旨 |
The p53 tumor suppressor is a tetrameric transcription factor. Genome-wide analysis of its binding sites in normal human IMR-90 fibroblasts revealed 743 high-confidence ChIP-seq peaks representing putative p53 binding sites. More than 40% were located within 2 kb of a transcription start site (TSS), a distribution similar to that documented for individually studied, functional p53 response elements. Nearly half of the peaks reside in CpG islands, in marked contrast to sites reported in cancer-derived cells. The distinct genomic features of the IMR-90 binding sites do not reflect a distinct preference for specific sequences since the de novo developed p53 motif based on our study is similar to those reported by genome-wide studies of cancer-derived cells. More likely, the different chromatin landscape in normal compared to cancer-derived cells influences p53 binding via modulating the availability of sites through epigenetic mechanisms. We compared the IMR-90 ChIP-seq peaks to the recently published IMR-90 methylome and demonstrate that they are enriched at hypomethylated DNA. A hypothesis for the differences in p53 binding between normal human cells and cancer-derived cell lines will be presented.
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| 主催 |
グローバルCOE物質科学イノベーション講演会 |
| 共催 |
日本生化学会北海道支部
生命分子化学セミナー
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| 連絡先 |
坂口和靖
北海道大学大学院理学研究院化学部門
札幌市北区北10条西8丁目
TEL: 011-706-2698, FAX: 011-706-4683
e-mail: kazuyasu@sci.hokudai.ac.jp
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